Hi Santarem01,
Thank you for your question.
If we could access owa on Exch02?
If we could access ECP by https://localhost/ecp on Exch02?
Did you try to access https://exchange01/ecp when exch02s IIS was disabled?
We could make sure there are same configuration between Exch01 and Exch02 in IIS in term of authentication.
Then we could restart IIS on Exch02.
If the issue persist, we could rebuild ECP virtual Directory by the following command:
Remove-EcpVirtualDirectory -Identity "Server02\ecp (default Web site)"
New-EcpVirtualDirectory -Identity "Server02\ecp (default Web site)"
After we rebuild ECP virtual directory, we must restart IIS by running iisreset /noforce from a command prompt window.
If there are any questions regarding this issue, please be free to let me know.
Best Regard,
Jim